Calgary Mayor Jyoti Gondek says the move is an insult to Calgarians who are already struggling to get by.
The Calgary program works on a sliding scale with those most in need paying as little as $5.80 a month for a transit pass.
A regular adult monthly pass, by comparison, costs $115.
Gondek says the city saw more than 119,000 low-income passes issued in the first three months of this year to aid those living below the poverty line.
Edmonton Mayor Amarjeet Sohi says defunding the program will make life more expensive for 25,000 local residents every month, including students and seniors.